{"id":251,"date":"2016-01-27T15:07:00","date_gmt":"2016-01-27T15:07:00","guid":{"rendered":"http:\/\/www.efolio.soton.ac.uk\/blog\/discoveroceanography\/?page_id=251"},"modified":"2019-06-08T12:46:43","modified_gmt":"2019-06-08T11:46:43","slug":"sampling-techniques","status":"publish","type":"page","link":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/for-teachers\/workbook-2\/sampling-techniques\/","title":{"rendered":"Sampling techniques"},"content":{"rendered":"<h1><a name=\"_Toc309119712\"><\/a>Why do we <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span>, <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span>, net and grab?<\/h1>\n<p>To see what is living in the water column, above the seabed as well as on and in the seabed. Whilst diving can provide us with a direct view of the visible animals and plants growing on the seabed, it is a specialist and very time consuming activity that can only be used in relatively shallow water (say 0-30m). Diving is used if the scientific objectives gained from a \u2018diver\u2019s eye view\u2019 justify the time and cost involved. For routine <span class='tooltipsall tooltipsincontent classtoolTips41'>Sampling<\/span>, or <span class='tooltipsall tooltipsincontent classtoolTips41'>Sampling<\/span> in places where diving is inappropriate, trawls nets and grabs can provide the samples we need to see what is living in the <span class='tooltipsall tooltipsincontent classtoolTips71'>Ocean<\/span> and where it can be found.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-299 size-medium aligncenter\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/Trawl-2-300x200.jpg\" alt=\"\" width=\"300\" height=\"200\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/Trawl-2-300x200.jpg 300w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/Trawl-2-768x511.jpg 768w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/Trawl-2.jpg 1000w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<h1><a name=\"_Toc309119713\"><\/a>Trawling<\/h1>\n<p>A beam <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span>, as the name implies, is a <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span> where the mouth is held open by a metal beam. The beam is mounted on <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span> heads or skids, one at each side of the net. The skids slide across the sea bed dragging the ground rope and net behind. The ground rope runs in an arc behind the skids and has weights attached, to hold it down against the seabed and agitate the fish, which hopefully attempt to swim away and get caught in the net. Commercially this gear is used primarily for flatfish. Several lengths of tickler chains will often be used ahead of the ground rope to raise fish which may otherwise be over-run.<\/p>\n<p>This type of net is designed to catch fish living on or near the seabed (demersal <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span>) but will sometimes catch fish that normally live in the main body of water (pelagic <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span>). In addition the net will catch some benthic <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> such as shell fish, seaweeds and crustacean. Catching fish in this way allows scientists to know what <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> are living in which areas of the estuary, and because the trawls are short and the water shallow, most fish survive and can be returned to the water after examination.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-298 size-medium aligncenter\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-1-300x255.jpg\" alt=\"\" width=\"300\" height=\"255\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-1-300x255.jpg 300w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-1-768x653.jpg 768w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-1.jpg 887w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>By <span class='tooltipsall tooltipsincontent classtoolTips41'>Sampling<\/span> at different times of year we can measure growth of individuals within a population, by tagging and re-catching fish we can estimate population size, growth rates and also discover how far the fish travel away from their original point of capture. We can also look at detailed aspects of the fish\u2019s life cycle, when they reproduce and what they feed on, to give us a better understanding of the way the sea supports its fish populations.<\/p>\n<p>Often during the summer months there will be large volumes of seaweed in the water, which quickly fill the beam <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span> net and make it very heavy and difficult to recover. During these periods it is often easier and safer to use an alternative method to collect samples. One alternative is to use an oyster <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span>.<\/p>\n<h1><a name=\"_Toc309119714\"><\/a>Dredging<\/h1>\n<p>Dredges are designed to be pulled behind a boat and to scrape along the seabed, the catch being held in mesh bag behind the mouth of the <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span>. Dredging is used to harvest commercial shellfish like oysters, clams and cockles and specialist designs have been developed for different uses.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-300 size-medium aligncenter\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-300x242.png\" alt=\"\" width=\"300\" height=\"242\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-300x242.png 300w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl-768x619.png 768w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/trawl.png 894w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>An oyster <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> is designed primarily to catch oysters but will catch other shellfish and benthic <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span>. We use two types, a traditional \u2018A\u2019 frame oyster <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> and a modern \u2018ladder\u2019 <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span>, as used commercially in the Solent for Oyster fishing. Dredges aren\u2019t very selective so even when targeting oysters we will catch a variety of the animals and plants living on the seabed. An oyster <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> will sometimes catch small demersal fish but this is usually as a result of good fortune rather than design.<\/p>\n<p>As the picture above shows the \u2018A\u2019 frame oyster <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> is a net held onto a triangular frame. The bottom edge of the triangle is a flat metal plate designed to dig into the seabed surface. This allows collection of whatever is disturbed, in the net. The ladder <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> is a more robust <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> and has a strong metal mesh that holds the catch.<\/p>\n<p>The other <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> that can be used is one designed to harvest clams from the muddy parts of Southampton Water. This <span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> is very heavy and digs right into the seabed to remove the clams.One of the good things about oysters is that their shell provides a <span class='tooltipsall tooltipsincontent classtoolTips53'>Habitat<\/span> for lots of other <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> so we are surveying not just oysters but lots of other <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> as well!<\/p>\n<h1><a name=\"_Toc309119717\"><\/a>How do you quantify <span class='tooltipsall tooltipsincontent classtoolTips67'>Trawl<\/span>\/<span class='tooltipsall tooltipsincontent classtoolTips59'>Dredge<\/span> <span class='tooltipsall tooltipsincontent classtoolTips41'>sample<\/span>?<\/h1>\n<h2><a name=\"_Toc309119715\"><\/a>Grabs<\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-302  alignleft\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/grab-diagram-1-212x300.png\" alt=\"\" width=\"227\" height=\"321\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/grab-diagram-1-212x300.png 212w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/grab-diagram-1-768x1087.png 768w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/grab-diagram-1-724x1024.png 724w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/grab-diagram-1.png 1533w\" sizes=\"auto, (max-width: 227px) 100vw, 227px\" \/>These allow us to discover what is living in the seabed by \u2018grabbing\u2019 a chunk of the mud and bringing it up to the surface. We often use a Van Veen grab (named after its inventor) which works well in mud. On<br \/>\nce the <span class='tooltipsall tooltipsincontent classtoolTips41'>sample<\/span> is aboard we can investigate the mud, looking at the size of the mud particles and also for signs of <span class='tooltipsall tooltipsincontent classtoolTips32'>Pollution<\/span>. We can wash the animals out onto a sieve and then count and identify them.<\/p>\n<p>The animals that live in the mud are known as infauna, and changes in the infaunal <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> composition are often used to judge if there have been any changes in environmental quality. Marine scientists use changes in number and <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> in the infaunal community to judge if there have been negative effects from say, a <span class='tooltipsall tooltipsincontent classtoolTips32'>Pollution<\/span> event, or if disturbance from fishing or dredging activity is harming the marine environment<\/p>\n<h2><a name=\"_Toc309119716\"><\/a>Plankton Nets<\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-303  alignleft\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets-1-225x300.jpg\" alt=\"\" width=\"170\" height=\"227\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets-1-225x300.jpg 225w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets-1.jpg 591w\" sizes=\"auto, (max-width: 170px) 100vw, 170px\" \/>Though many plankton <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> are microscopic, plankton includes plants and animals of a wide <span class='tooltipsall tooltipsincontent classtoolTips68'>Range<\/span> of sizes, such as jelly fish, which can grow many metres in length. The plankton is made up of animals (<span class='tooltipsall tooltipsincontent classtoolTips23'>Zooplankton<\/span>) and plants (<span class='tooltipsall tooltipsincontent classtoolTips12'>Phytoplankton<\/span>) that live in the water column and passively drift with the flow of the water. Many animals, like oysters and clams, have larval stages that live in the plankton for a few weeks and then settle onto the seabed. These animals, which are only plankton for part of their life are known as meroplankton. There are many other animals, like copepods, that live their whole life as plankton. These are known as holoplankton.<\/p>\n<p>As many of the planktonic animals and plants are very small, we need to use nets with very small holes (between 0.002 and 0.0006 mm) in them. These \u2018sieve\u2019 the plankton from the water, as they are towed slowly through the water. These are collectively known as plankton nets<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-304  alignright\" src=\"http:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets2-300x207.jpg\" alt=\"\" width=\"285\" height=\"197\" srcset=\"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets2-300x207.jpg 300w, https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-content\/uploads\/sites\/335\/2016\/01\/nets2.jpg 749w\" sizes=\"auto, (max-width: 285px) 100vw, 285px\" \/>Plankton nets are long conical nets, often used with a flow meter at the mouth, to measure how much water goes through the net and sometimes a <span class='tooltipsall tooltipsincontent classtoolTips72'>Depth<\/span> sensor is also attached. By <span class='tooltipsall tooltipsincontent classtoolTips41'>Sampling<\/span> plankton we can just look at the <span class='tooltipsall tooltipsincontent classtoolTips68'>Range<\/span> of <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> present. This is important because long term changes in the plankton can change the food web available to other animals and may even change the distribution of fish, like herring which prefer certain plankton <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> to eat. By focusing on a particular group or <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> we can see if they are producing enough larvae for the local population to survive. Quite often we will be looking for the larval stages of a commercial <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> such as oysters. In areas such as\u00a0 the Solent, where oyster fishing is in decline, the presence of larvae in the water is a good sign that the oysters are fit enough to reproduce.<\/p>\n<p><strong>Have a go at labelling the various <span class='tooltipsall tooltipsincontent classtoolTips41'>Sampling<\/span> tools on R.V. Callista below:<\/strong><\/p>\n<div class=\"h5p-iframe-wrapper\"><iframe id=\"h5p-iframe-12\" class=\"h5p-iframe\" data-content-id=\"12\" style=\"height:1px\" src=\"about:blank\" frameBorder=\"0\" scrolling=\"no\"><\/iframe><\/div>\n<script type=\"text\/javascript\"> toolTips('.classtoolTips0','The variable that is changed or controlled in a scientific experiment to test the effects on\u00a0the dependent variable.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips12','Plankton consisting of microscopic plants.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips20','The branch of science that deals with the physical and biological properties and phenomena of the sea.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips23','Plankton consisting of small animals and the immature stages of larger animals.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips32','<span style=\"font-family: verdana, geneva, sans-serif\">The presence in or introduction into the environment of a substance which has harmful or poisonous effects.<\/span>'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips41','Taking a sample or samples of (something) for analysis.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips41','Taking a sample or samples of (something) for analysis.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips46','A minute portion of matter.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips51','A small or microscopic aquatic crustacean of the large class\u00a0<i>Copepoda<\/i>.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips52','A group of living organisms consisting of similar individuals capable of exchanging genes or interbreeding. The <span class='tooltipsall tooltipsincontent classtoolTips52'>Species<\/span> is the principal natural taxonomic unit, ranking below a genus and denoted by a Latin binomial, e.g.\u00a0<i>Homo sapiens<\/i>.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips53','The natural home or environment of an animal, plant, or other organism.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips59','To clear the bed of (a harbour, river, or other area of water) by scooping out mud, weeds, and rubbish with a dredge.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips62','<span style=\"font-family: verdana, geneva, sans-serif\">A body\\'s relative <span class='tooltipsall tooltipsincontent classtoolTips75'>Mass<\/span> or the quantity of matter contained by it, giving rise to a downward force; the heaviness of a person or thing.<\/span><br\/><br\/>&nbsp;<br\/><br\/><span style=\"font-family: verdana, geneva, sans-serif\">The force exerted on the <span class='tooltipsall tooltipsincontent classtoolTips75'>Mass<\/span> of a body by a gravitational field.<\/span>'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips63','The amount of space that a substance or object occupies, or that is enclosed within a container.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips67','An act of fishing with a trawl net or seine.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips68','<span style=\"font-family: verdana, geneva, sans-serif\">The <span class='tooltipsall tooltipsincontent classtoolTips74'>Area<\/span> of variation between upper and lower limits on a particular scale.<\/span>'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips71','A very large expanse of sea, in particular each of the main areas into which the sea is divided geographically.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips72','The distance from the top or surface to the bottom of something.'); <\/script><script type=\"text\/javascript\"> toolTips('.classtoolTips74','The amount of space inside the boundaries of a 2D or 3D object.'); <\/script>","protected":false},"excerpt":{"rendered":"<p>Why do we Trawl, Dredge, net and grab? To see what is living in the water column, above the seabed as well as on and in the seabed. Whilst diving can provide us with a direct view of the visible animals and plants growing on the seabed, it is a &hellip;<\/p>\n","protected":false},"author":3738,"featured_media":1463,"parent":114,"menu_order":2,"comment_status":"closed","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-251","page","type-page","status-publish","has-post-thumbnail","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/pages\/251","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/users\/3738"}],"replies":[{"embeddable":true,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/comments?post=251"}],"version-history":[{"count":3,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/pages\/251\/revisions"}],"predecessor-version":[{"id":1683,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/pages\/251\/revisions\/1683"}],"up":[{"embeddable":true,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/pages\/114"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/media\/1463"}],"wp:attachment":[{"href":"https:\/\/generic.wordpress.soton.ac.uk\/discoveroceanography\/wp-json\/wp\/v2\/media?parent=251"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}